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Evaluation of Costus afer Ker Gawl. In vitro anti-inflammatory activity and its chemical constituents identified using gas chromatography-mass spectrometry analysis.

Authors: ANYASOR Godswill , Onajobi Funmilayo , Osilesi Odutola , Adebawo , Efere Martins Oboutor

Publication Type: Journal article

Journal: Journal Of Coastal Life Medicine

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Abstract

Objective: To evaluate the anti-inflammatory activity of a tropical African medicinal plant,
Costus afer (C. afer) Ker Gawl. in vitro and identify the chemical constituents in the most
active fraction.
Methods: Hexane, ethyl acetate, n-butanol and aqueous fractions obtained through successive
solvent partitioning of 70% methanolic leaf or stem extracts of C. afer were subjected to
in vitro anti-inflammatory screening assays viz. anti-denaturation of protein, stabilization
of human red blood cell (HRBC) membrane against hypotonicity-induced hemolysis and
anti-proteinase activities. Diclofenac sodium was used as a standard drug. The chemical
compounds in the most active fraction were determined using quantitative phytochemical and
gas chromatography-mass spectrometry (GC/MS) analytical methods, comparing the mass
spectra of the GC/MS identified compounds with those of the National Institute of Standards
and Technology (NIST) database library.
Results: The hexane fraction of C. afer leaf (HFCAL) with an IC50 of 33.36 ?g/mL, 33 ?g/mL
and 212.77 ?g/mL exhibited the highest anti-denaturation of protein, stabilization of HRBC
membrane and anti-proteinase activities respectively when compared with other test fractions.
The GC/MS identified compounds in HFCAL known to possess anti-inflammatory property
were terpenoids (naphthalene 1,6-dimethyl-; naphthalene 2,3-dimethyl-; phytol), phenol
[phenol 2,4-bis(1,1-dimethylethyl)], coumaran [2(4H)-benzofuranone, 5,6,7,7a-tetrahydro-4,
4,7atrimethyl and fatty acids (pentadecanoic acid; hexadecanoic acid; n-hexadecanoic acid;
9,12-octadecanoic acid-methyl ester; 9,12,15 octadecatrienoic acid and cis-vaccenic acid)].
Conclusion: Therefore, HFCAL could be considered as a potential source of antiinflammatory
agents for herbal formulation or pharmaceutical drug production.

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ANYASOR,G. Onajobi,F. Osilesi,O. Adebawo,O. Efere,M.O . (2015). Evaluation of Costus afer Ker Gawl. In vitro anti-inflammatory activity and its chemical constituents identified using gas chromatography-mass spectrometry analysis., 3 (), 132-132.

ANYASOR,G. Onajobi,F. Osilesi,O. Adebawo,O. Efere,M.O . "Evaluation of Costus afer Ker Gawl. In vitro anti-inflammatory activity and its chemical constituents identified using gas chromatography-mass spectrometry analysis." 3, no (), (2015): 132-132.

ANYASOR,G. and Onajobi,F. and Osilesi,O. and Adebawo,O. and Efere,M.O and . (2015). Evaluation of Costus afer Ker Gawl. In vitro anti-inflammatory activity and its chemical constituents identified using gas chromatography-mass spectrometry analysis., 3 (), pp132-132.

ANYASORG, OnajobiF, OsilesiO, AdebawoO, EfereMO, . Evaluation of Costus afer Ker Gawl. In vitro anti-inflammatory activity and its chemical constituents identified using gas chromatography-mass spectrometry analysis.. 2015, 3 ():132-132.

ANYASOR,Godswill , Onajobi,Funmilayo , Osilesi,Odutola , Adebawo,Olugbenga , and Efere,Martins Oboutor . "Evaluation of Costus afer Ker Gawl. In vitro anti-inflammatory activity and its chemical constituents identified using gas chromatography-mass spectrometry analysis.", 3 . (2015) : 132-132.

[1] A.Godswill O.Funmilayo O.Odutola A.Olugbenga & E.Martins Oboutor , "Evaluation of Costus afer Ker Gawl. In vitro anti-inflammatory activity and its chemical constituents identified using gas chromatography-mass spectrometry analysis." vol.3, no., pp. 132-132, 2015.

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