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Anti-inflammatory properties and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves.

Authors: ANYASOR Godswill

Publication Type: Journal article

Journal: American Journal Of Physiology, Biochemistry And Pharmacology

ISSN Number:

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Abstract

Aim: This study was designed to determine the most active anti-inflammatory fraction of
Crateva adansonii leaves and characterize the bioactive compounds.
Methods: Dried and pulverized C. adansonii leaves (CAL) were extracted with 70% methanol,
followed by successive solvent partitioning into hexane, ethyl acetate (CALE), butanol,
and aqueous leaf fractions. In vitro antioxidant assays carried out on the extract/
fractions were 1,1-diphenyl 2-picryl hydrazyl (DPPH), hydrogen peroxide, ferric reducing
antioxidant power (FRAP), nitric oxide scavenging, total antioxidant capacity (TAC),
total phenol, and flavonoid (TF) assays. In vitro anti-inflammatory assays investigated
were heat-induced bovine serum albumin (BSA) denaturation and human red blood cell
(HRBC) membrane stabilization against hypotonicity-induced hemolysis. Formaldehydeinduced
inflammation model in rats was carried out, followed by paw edema measurement,
as well as serum alanine aminotransferase (ALT) and aspartate aminotransferase
(AST) activities assays. The CALE fraction was subjected to gas chromatography-mass
spectrophotometry (GC-MS) analytical method.
Results: CAL extract and fractions substantially inhibited BSA denaturation and stabilized
HRBC membrane against hypotonicity-induced hemolysis; however, CALE exhibited the
highest activity. CALE also suppressed formaldehyde-induced rat paw edema and significantly
(p < 0> significantly high (p < 0> activities when compared with the other test samples. CALE also exhibited an appreciable
amount in FRAP, TAC, and TF. GC-MS analysis of CALE indicated the presence of 19
tentatively identified compounds with anti-inflammatory and/or antioxidant properties.
Conclusion: Ethyl acetate fraction of C. adansonii leaves exhibited anti-inflammatory
activities which could be through antioxidant effect and perhaps through suppression
of pro-inflammatory mediators. Hence, ethyl acetate fraction of C. adansonii leaf could
serve as an important source of a lead drug candidate for pharmaceutical drug development
in the management of inflammation.

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ANYASOR,G. . (2019). Anti-inflammatory properties and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves., 9 (), 9-9.

ANYASOR,G. . "Anti-inflammatory properties and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves." 9, no (), (2019): 9-9.

ANYASOR,G. and . (2019). Anti-inflammatory properties and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves., 9 (), pp9-9.

ANYASORG, . Anti-inflammatory properties and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves.. 2019, 9 ():9-9.

ANYASOR,Godswill , . "Anti-inflammatory properties and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves.", 9 . (2019) : 9-9.

[1] A.Godswill , "Anti-inflammatory properties and gas chromatography-mass spectrometry analysis of ethyl acetate fraction of Crateva adansonii DC leaves." vol.9, no., pp. 9-9, 2019.

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