Babcock University Research Portal

Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models.

Authors: ANYASOR Godswill

Publication Type: Journal article

Journal: Clinical Phytoscience

ISSN Number:

Downloads

Views

Abstract

Background: Justicia secunda Vahl. is a medicinal plant used in ethnomedical practice as therapy to manage
inflammation. Therefore, this study was designed to evaluate the anti-inflammatory activity of methanol extract of J.
secunda leaves (MEJSL) using in vitro and in vivo inflammation models.
Methods: Seventy-percent MEJSL was prepared following standard procedure. In vitro anti-inflammatory assays
were performed using heat-induced bovine serum albumin (BSA) denaturation and erythrocyte membrane
stabilization assays. Carrageenan and formaldehyde induced inflammation in rat models were used to evaluate the
anti-inflammatory activity of MEJSL in vivo. Diclofenac sodium was used as a reference drug. In addition, liver and
kidney function assays and hematological analysis were carried out.
Results: Data revealed that varying concentrations of MEJSL significantly (P < 0> denaturation and stabilized erythrocyte membrane against hypotonicity-induced hemolysis when compared with
diclofenac sodium in a concentration-dependent manner. In vivo study showed that 10 mg/kg body weight (b.w.)
diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL suppressed carrageenan-induced paw edema at the sixth hour
by 71.14%, 83.08%, and 89.05%, respectively. Furthermore, 10 mg/kg b.w. diclofenac sodium, 100 and 300 mg/kg
b.w. MEJSL inhibited formaldehyde-induced paw edema by 72.53%, 74.73%, and 76.48%, respectively. Animals
treated with varying doses of MEJSL had reduced plasma aspartate aminotransferase and alanine aminotransferase
activities; urea and creatinine concentrations; and modulated hematological parameters when compared with the
untreated control group.
Conclusions: Findings from this study showed that MEJSL exhibited substantial anti-inflammatory actions in the
in vitro and in vivo models. It also indicated that MEJSL anti-inflammatory mechanisms of action could be through
interference with phase 2 inflammatory stressors, upregulation of cytoprotective genes, stabilization of inflammatory
cell membranes and immunomodulatory activity.

Access to Documents

External Link
Download
Digital Object Identifier

Keywords

ANYASOR,G. . (2019). Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models., 49 (), 1-1.

ANYASOR,G. . "Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models." 49, no (), (2019): 1-1.

ANYASOR,G. and . (2019). Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models., 49 (), pp1-1.

ANYASORG, . Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models.. 2019, 49 ():1-1.

ANYASOR,Godswill , . "Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models.", 49 . (2019) : 1-1.

[1] A.Godswill , "Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models." vol.49, no., pp. 1-1, 2019.

Access to Documents

Keywords

About Us

Admissions

Contact Us

Policies

ALUMNI

STUDENT POPULATION